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Ferrets were divided randomly into four groups based upon SARS-CoV infection, mock infection, and challenge with both virus and mock inoculation. On 0 d.p.i., twentyfour ferrets (Group 1) were inoculated intranasally with serumfree medium only, and forty-two (Group 2) with SARS-CoV (103 TCID50/mL) diluted in medium. On 29 d.p.i., nine ferrets (Group 3) were inoculated with serum-free medium, and eighteen ferrets (Group 4) were challenged and inoculated with SARS-CoV (10^3 TCID50) diluted in medium. Prior to instillation of mock or virus inoculums, all ferrets were anesthetized,and 1 mL of the inoculum was administered intranasally by drop-wise delivery of 500 μL per nare.

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轻度支气管炎